t4 ligase promega protocol – promega t4 ligase protocol
A protocol for use with T4 Polynucleotide Kinase to transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 3´-phosphate group, T4 PNK is widely used to end-label short oligonucleotide probes, DNA and RNA molecules,
T4 DNA Ligase
T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides, The enzyme will not join single-stranded nucleic acids, T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl pH 7,8 at 25°C, 100mM MgCl 2, 100mM DTT and 10mM ATP,
T4 DNA Ligase, Blue/White Cloning Qualified Protocol, T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration, The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex
T4 RNA Ligase Protocol
T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5′-phosphoryl termini of single-stranded RNA or DNA, The enzyme, purified from recombinant Escherichia coli CA4 RNase I-deficient, has an apparent molecular weight of 43,5kDa, Revised 4/18,
T4 DNA Ligase Blue/White Cloning Qualified Protocol
Protocol Set up the following reaction in a microcentrifuge tube on ice, T4 DNA Ligase should be added last, Note that the table Gently mix the reaction by pipetting up and down and microfuge briefly, For cohesive sticky ends, incubate at 16°C overnight or room temperature for 10 minutes, For
T4 DNA Ligase, Blue/White Cloning Qualified Protocol, T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration, The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex
1, ®Use only the T4 DNA Ligase supplied with this system to perform pGEM -T and pGEM®-T Easy Vector ligations, Other commercial preparations of T4 DNA ligase may contain exonuclease activities that may remove the terminal deoxythymidines from the vector, 2, 2X Rapid Ligation Buffer contains ATP, which degrades during temperature fluctuations, Avoid multiple
T4 DNA Ligase
2, The ligation conditions given in this protocol are based on the conditions used at Promega for quality control of lambda vectors with sticky ends, These ligation condi-tions have been developed using Promega Blue/White Cloning-Qualified T4 DNA Ligase, 3, The addition of polyethylene glycol PEG to ligation reactions can promote ligation of
T4 DNA Ligase Product Information Sheet 9PIM180
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T4 DNA Ligase
T4 RNA Ligase Protocol
Ligation Protocol with T4 DNA Ligase M0202
2 The ligation conditions given in this protocol are based on the conditions used at Promega for quality control of lambda vectors with sticky ends These ligation condi-tions have been developed using Promega Blue/White Cloning-Qualified T4 DNA Ligase 3, The addition of polyethylene glycol PEG to ligation reactions can promote ligation of
T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides, The enzyme will not join single-stranded nucleic acids, T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl pH 7,8 at 25°C, 100mM MgCl 2, 100mM DTT and 10mM ATP,
T4 DNA Ligase
T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides, The enzyme will not join single-stranded nucleic acids, T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl pH 7,8 at 25°C, 100mM MgCl 2, 100mM DTT and 10mM ATP,
T4 Polynucleotide Kinase Protocol
T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides, The enzyme will not join single-stranded nucleic acids, T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl pH 7,8 at 25°C, 100mM MgCl 2, 100mM DTT and 10mM ATP,
pGEM -T and pGEM -T Easy Vector Systems
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t4 ligase promega protocol
T4 DNA Ligase Blue/White Cloning Qualified Protocol
T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5′-phosphoryl termini of single-stranded RNA or DNA, The enzyme, purified from recombinant Escherichia coli CA4 RNase I-deficient, has an apparent molecular weight of 43,5kDa, Revised 4/18,
Part# 9PIM180 Revised 4/18
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